Seed: FHRD S1N1 (normal) Bomb deals 1 damage. Die to your own bombs in The Caves or Catacombs as Magdalene. Make sure to have have half heart when you run in. Mulliboom is in the room under the spawn room. Die to a Mulliboom in the Basement or Cellar as Isaac. Isaac starts with the compass, treasure map and the missing poster.

Unlocking before the DLC: The Lost is unlocked via very specific deaths which must be done in order without dying erroneously between runs. This achievement is earned when you donate 879 coins on the donation machine that appears at the end of greed mode when you beat the final boss. Die to the 2nd stage of the Satan boss fight as Azazel.

After completing the first level, beat any stage while at 1/2 heart of health OR while playing as The Lost. The Noose: Defeat boss in The Womb 2 2nd time. In Afterbirth all you need to do unlock The Lost is die to the spikes in a Sacrifice room while having the Missing Poster trinket.

Judas: Defeat boss in The Womb 2 1st time. The Lost's unlocks can be a bit easier to unlock … The lost can be unlocked with seeded runs at any step of the process except for the final one. UNLOCK: Unlock this item by picking up Rubber Cement 5 times. 7 Isaac must die from a Mulliboom in the basement/cellar. The lost can be unlocked with seeded runs at any step of the process except for the final one. Instead of generating random dungeons, you’ll unlock cheat-like … This effect will only work once and will negate the effect of the next health increasing item the player picks up.

How to unlock the lost rebirth